Facilities

New facilities for the research activities of the research infrastructure were built as an extension of the existing pavilion “V” at Institute of Molecular Genetics ASCR, v. v. i. (IMG), creating a single integrated unit, including facilities needed for the functioning of the infrastructure. The facility consists of 5 laboratories and 3 tissue culture laboratories with a total area of 500 m2. Other supporting parts of the infrastructure are situated in the building F (the main building of the IMG), where research and service laboratories are located (core facilities of IMG). In addition, state-of-the-art animal facilities (mouse, chicken and zebrafish) at IMG can be easily accessed for individual projects to in vivo validate compound activities and/or their toxicity.

CZ-OPENSCREEN is unique infrastructure in the Czech Republic due to its state-of-the-art equipment, know-how and multidisciplinary approach. It gives users in the CR opportunity to perform cutting-edge research. It is achieved by employment of the stand-alone readers or fully integrated instruments into automatic robotic units (integrated robotic HTS station for screening of chemical compounds, robotic integrated system for automated microscopic analysis and label-free technology, Integrated system for compound storage and sample preparation). Users from both the CR and abroad take advantage of the full spectrum of devices and instruments available, advanced liquid handling (including acoustic transfer), compound management (access to compound library, cherry-picking of active compounds).

Besides the basic readouts of absorbance, fluorescence, luminescence, radioactivity and image-based, unique measurement technologies available at the RI include measurements of Ca2+ mobilization and cAMP in cells (FLIPR and aequorin assays), AlphaScreen, time-resolved fluorescence (TRF), fluorescence polarization, label-free technologies (impedance – xCELLigence and dynamic mass redistribution - EPIC). Another unique aspect is that we perform assays in 96-, 384- or 1536-well formats.