Knime4HTS is a set of new nodes and prepared workflows designed for the processing of HTS data via the Knime Analytics Platform. Using these tools, raw plate data in a matrix or column coordinate format can be analyzed. KNIME4HTS provides functionality for raw data normalization, hit selection, the visualization of plate heatmaps and generation of screening reports. Prepared workflows are also able to visualize large chemical libraries by means of chemical space according to their physico-chemical properties and structural features.